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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP2105b
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WB, IHC, E, IF†
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H
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RB4107/4108
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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Q9HAU4
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SMURF2.
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Other Names:
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E3 ubiquitin-protein ligase SMURF2; Smad ubiquitination regulatory factor 2; Smad-specific E3 ubiquitin ligase 2;
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Application Data:
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Calculated MW:
86113 Da
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Western blot analysis of anti-SMURF2 Pab (Cat. #AP2105b) in 293 cell line lysate (35ug/lane). SMURF2(arrow) was detected using the purified Pab (1:30 dilution) |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Product Citations:
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- Schwamborn, Jens C., et al. J. Biol. Chem. 2007 Nov 30;282(48):35259-35268. (Applications: IF)
- Gabriele Sass, et al. J. Immunol., Nov 2007; 179: 7042 - 7049. .
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
† Application validated by third-party citation.
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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