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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP2162a
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WB, IHC, E
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H More
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RB4403/4404
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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P26442
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AMFR.
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Other Names:
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Autocrine motility factor receptor precursor, isoform 1; AMF receptor
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Application Data:
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Calculated MW:
34325 Da
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(LEFT)Western blot analysis of anti-AMFR Antibody (N-term) (Cat. #AP2162a) in CEM cell line lysate (35ug/lane). AMFR (arrow) was detected using the purified Pab.
(RIGHT)Western blot analysis of AMFR (arrow) using AMFR Antibody (N-term) (Cat.#AP2162a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMFR gene (Lane 2) (Origene Technologies). |
(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
(RIGHT)Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AMFR Antibody (N-term) (Cat.#AP2162a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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- Kenichiro Araki, et al. J. Biol. Chem. 2009; 284:32305-32311. (E-pub)
- Kenichiro Araki, et al. J. Biol. Chem. 2009; 284:32305-32311.
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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