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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP2183b
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WB, IF, IHC, E
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H, (M) More
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RB4614
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_003891, Q13501
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SQSTM1.
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Other Names:
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Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, ORCA, OSIL
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Application Data:
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Calculated MW:
47687 Da
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(LEFT) Western blot analysis of hSQSTM1-C331 (Cat. #AP2183b) in A2058 cell line lysates (35ug/lane). SQSTM1 (arrow) was detected using the purified Pab.
(RIGHT) Western blot analysis of SQSTM1 (arrow) using rabbit polyclonal Autophagy SQSTM1 (p62) Antibody (C-term ) (Cat.#AP2183b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2) (Origene Technologies). |
TOP: Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. BOTTOM: Immunofluorescence staining of Autophagy SQSTM1 (p62) Antibody (C-term) (Cat# AP2183b) on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland. |
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Product Citations:
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- Michal Caspi Tal, et al. PNAS, Feb 2009; 10.1073/pnas.0807694106. (Applications: WB)(E-pub)
- Jeong-Sun Ju, et al. J. Biol. Chem., Oct 2008; 283: 30289 - 30299. (Applications: IF)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:50~100 |
| Immunofluorescence | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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