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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP2507b
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WB, IHC, E
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H
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RB2575
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_002219, P05412
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide HPRLQALKEEPQTVPE containing a predicted sumoylation site from the C-terminal region of human AP-1.
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Other Names:
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Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUN
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Application Data:
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Calculated MW:
35675 Da
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(LEFT)The AP-1 C-term polyclonal antibody (Cat. #AP2507b) is used in Western blot to detect nonsumoylated AP-1 in Jurkat cell lysate.
(RIGHT)Western blot analysis of AP-1(arrow) using rabbit polyclonal AP-1 Antibody (C-term) (RB02575). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AP-1 gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Product Citations:
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Data Rewards Program
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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