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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP3001a
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WB, IHC, E
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H
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RB7159
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_006752, P62258
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T232 of human 14-3-3.
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Other Names:
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14-3-3 protein epsilon; 14-3-3E; YWHAE;
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Application Data:
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Calculated MW:
29174 Da
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(Top)The anti-YWHAZ Pab (Cat. #AP3001a) is used in Western blot to detect YWHAZ in HL60 (left) and mouse brain (right) tissue lysates.
(Buttom) Dot blot analysis of anti-14-3-3-pT232 Pab (RB07159) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Product Citations:
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Data Rewards Program
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot BLot | 1:500 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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