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Phospho-H3-S28 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)


specificationbackgroundprotocolsprintmsds
 
Catalog # Applications: Reactivity: Clone Name:
AP3007a WB, E H RB5201
Concentration: Size: Accession: Isotype:
0.25 mg/ml 0.1 mg NP_003528, Q71DJ3 Rabbit Ig
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S28 of human H3.
Other Names:
Histone H3; H3 histone family; member K; H3 histone family, member I; H3 histone family, member L; HIST1H3A protein; HIST1H3F protein; HIST1H3E protein; HIST1H3B; HIST1H3A; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J;
Application Data:   Calculated MW:   15404 Da
     

Western blot analysis of anti-Phospho-H3-pS28 Pab (Cat. #AP3007a) in HepG2 cell line lysate (35ug/lane). Mouse Phospho-H3-pS28(arrow) was detected using the purified Pab (1:64 dilution).

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Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

 

 

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in stockList Price (USA): $235.00/0.1mg

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Abgent is the original manufacturer of this product.
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