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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP3019a
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WB, IHC, E
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H
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RB7127
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_005154, P31749
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S124 of human AKT1.
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Other Names:
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RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; Protein kinase B; PKB; C-AKT; AKT1; PKB; RAC;
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Application Data:
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Calculated MW:
55686 Da
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(TOP) Western blot analysis of anti-AKT1 Pab (Cat. #AP3019a) in A375 cell line lysate.AKT1(arrow) was detected using the purified Pab.
(BOTTOM) Phospho-Akt-S124 Catalog # AP3019a was used to detect Phospho-Akt in HUVEC. Human umbilical vein endothelial cells (HUVEC) were starved overnight and stimulated with either 50ng/ml of bFGF or VEGF for 5 min. Lysates were collected and SDS-PAGE was performed on a 10% gel. Data and protocol kindly provide by Dr. Eric Murphy of David Cheresh Lab, UCSD. |
(TOP) Western blot analysis of anti-Phospho-AKT1 Pab (Cat. #AP3019a) transiently transfected HEK-293 cell line lysate (1ug/lane). Phoshop-AKT1 pab(arrow) was detected using the purified Pab (1:1500 dilution).
(BOTTOM)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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