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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP3094a
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WB, DB, IHC, E
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H
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RB15813
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_004439, P04626
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1248 of human ErbB2.
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Other Names:
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Receptor tyrosine-protein kinase erbB-2 [Precursor]; p185erbB2; C-erbB-2; NEU proto-oncogene; Tyrosine kinase-type cell surface receptor HER2; MLN 19; ERBB2; HER2; NEU; NGL;
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Application Data:
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Calculated MW:
137910 Da
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(LEFT)Phospho-ErbB2-Y1248 Antibody (cat# AP3074a)was used to detect Phospho-ErbB2-Y1248 in FG pancreatic carcinoma cell line lysate.
FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min
Phospho-ErbB2-Y1248 Antibody (cat# AP3074a) was used at 1:750 in 3% BSA.
Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
(RIGHT)Dot blot analysis of anti-Phospho-ErbB2-pY1248(M) Antibody (Cat.#AP3094b) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Dot Blot | 1:500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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