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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP3251a
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DB, IHC, E
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H, (M) More
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RB7971
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_005350, Q13485
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T277 of human SMAD4.
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Other Names:
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Mothers against decapentaplegic homolog 4; SMAD 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; hSMAD4; SMAD4; DPC4; MADH4
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Application Data:
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Calculated MW:
60439 Da
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Dot blot analysis of anti-Phospho-SMAD4-T277 Antibody (RB7971) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
Formalin-fixed and paraffin-embedded Breast Carcinoma reacted with SMAD4-T277 (center) (Cat.#AP3251a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot Blot | 1:500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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