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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP3285a
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WB, IHC, DB, E
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H, (M) More
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RB7860
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_003381, P30291
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S53 of human WEE1
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Other Names:
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Wee1-like protein kinase; Wee1A kinase; WEE1hu; WEE1;
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Application Data:
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Calculated MW:
71597 Da
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(LEFT)The anti-Phospho-Wee1-S53 Pab (Cat. #AP3285a) is used in Western blot to detect Phospho-Wee1-S53 in mouse liver tissue lysate.
(RIGHT)Dot blot analysis of anti-Phospho-Wee1-S53 Antibody (Cat. #AP3285a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma |
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Product Citations:
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Data Rewards Program
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
| Dot Blot | 1:500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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