|
Catalog #
|
Applications:
|
Reactivity:
|
Clone Name:
|
|
AP3307a
|
WB, E
|
H
|
RB11167
|
|
Concentration:
|
Size:
|
Accession:
|
Isotype:
|
|
0.25 mg/ml
|
0.1 mg
|
NP_005219, P00533
|
Rabbit Ig
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1069 of human EGFR.
|
|
Other Names:
|
|
Epidermal growth factor receptor [Precursor]; Receptor tyrosine-protein kinase ErbB-1; EGFR; ERBB1;
|
|
Application Data:
|
|
Calculated MW:
134277 Da
|
|
|
|
|
Dot blot analysis of Phospho-EGFR-Y1069 polyclonal antibody (Cat# AP3307a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide. |
|
|
Product Citations:
|
|
Data Rewards Program
>
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|