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Phospho-MAP3K1-T1383 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)


specificationbackgroundprotocolsprintmsds
 
Catalog # Applications: Reactivity: Clone Name:
AP3321a WB, E H RB11308
Concentration: Size: Accession: Isotype:
0.25 mg/ml 0.1 mg NP_005912, Q13233 Rabbit Ig
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T1383 of human MAP3K1.
Other Names:
Mitogen-activated protein kinase kinase kinase 1; MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1; MAPKKK1; MEKK; MEKK1
Application Data:   Calculated MW:   164340 Da
     

Dot blot analysis of Phospho-MAP3K1-T1383 Pab (Cat.AP3321a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration were 0.5ug per ml.

Product Citations:
  1. Dariush Mokhtari, et al. Diabetes, Jul 2008; 57: 1896 - 1904.

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Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blot1:100~500
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

 

 

orderOrder Information

in stockList Price (USA): $235.00/0.1mg

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Abgent is the original manufacturer of this product.
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