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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP3373a
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WB, E
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H
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RB10946
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_004324, P15056
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S601 of human BRAF.
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Other Names:
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B-Raf proto-oncogene serine/threonine-protein kinase; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1; RAFB1
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Application Data:
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Calculated MW:
84437 Da
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Dot blot analysis of Phospho-BRAF-S601 Antibody (Cat. #AP3373a) and BRAF Non Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
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Product Citations:
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Data Rewards Program
>
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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