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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP5000e
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WB, IF, FC, E
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H
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RB18383
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_001207, Q16790
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CA9.
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Other Names:
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Carbonic anhydrase 9 [Precursor]; Carbonic anhydrase IX; Carbonate dehydratase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250
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Application Data:
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Calculated MW:
49698 Da
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Western blot analysis of CA9 antibody (Center) (Cat.# AP5000e) in Hela cell line lysates (35ug/lane). CA9 (arrow) was detected using the purified Pab. |
(LEFT) Immunofluorescence analysis of CA9 Antibody (Center) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
(RIGHT) Flow cytometric analysis of hela cells using CA9 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Product Citations:
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Data Rewards Program
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunofluorescence | 1:10~50 |
| Flow cytometric | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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