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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP6122a
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WB, FC, E
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H
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RB16781
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_848654, Q2M3G0
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ABCB5.
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Other Names:
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ATP-binding cassette sub-family B member 5; P-glycoprotein ABCB5; ABCB5 P-gp; ABCB5
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Application Data:
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Calculated MW:
89831 Da
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Western blot analysis of anti-ABCB5 Antibody (N-term) (Cat.#AP6122a) in K562 cell line lysates (35ug/lane). ABCB5(arrow) was detected using the purified Pab. |
TOP: Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# AP6122a on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody. Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
BOTTOM: Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# AP6122a on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody. Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver |
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Product Citations:
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Data Rewards Program
>
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Flow Cytometry | |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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