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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP6166a
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WB, IHC, E
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H More
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RB2196
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_002353, P43358
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MAGEA4.
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Other Names:
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Melanoma-associated antigen 4, MAGE-4 antigen, MAGE-X2, MAGE-41
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Application Data:
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Calculated MW:
34929 Da
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The anti-MAGE-A4 Pab (Cat. #AP6166a) is used in Western blot to detect MAGE-A4 in A375 cell lysate. |
TOP: Immunostaining of paraformldehyde-fixed and paraffin-embedded human breast carcinoma with MAGE-A4 antibody Cat# AP6166a (1:50 dilution), followed by reacting with biotin-conjugated secondary antibody, ABC solution and developing with DAB. Cancerous cells show nuclear staining of MAGE-A4. Magnification: 40X. Data courtesy of Dr. Mi Hou, Karolinska Institutet and
University Hospital, Sweden. BOTTOM: Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAGEA4 antibody (N-term) (Cat.#AP6166a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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Data Rewards Program
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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