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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP7028b
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WB, E
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H, (M) More
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RB3023-3024
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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P31749
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AKT1.
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Other Names:
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RAC-alpha serine/threonine kinase, RAC-PK-alpha, Protein kinase B, PKB, C-AKT
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Application Data:
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Calculated MW:
55716 Da
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(LEFT) Western blot analysis of hAKT1-D453 (Cat. #AP7028b) in Y79 cell line lysates (35ug/lane).AKT1 (arrow) was detected using the purified Pab.
(RIGHT) Western blot analysis of AKT1 (arrow) using rabbit polyclonal hAKT1-D453 (Cat. #AP7028b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT1 gene (Lane 2) (Origene Technologies). |
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Product Citations:
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- Yi Cai, et al. Cancer Res., Feb 2009; 69: 819 - 827.
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blot | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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