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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP7240a
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WB, IHC, E
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H, M, Pr, R More
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RB3534
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_004208, Q96GD4
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Rabbit Ig
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Target/Specificity:
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Other Names:
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Serine/threonine-protein kinase 12; Aurora-B; Aurora- and Ipl1-like midbody-associated protein 1; AIM-1; Aurora/IPL1-related kinase 2; Aurora-related kinase 2; STK-1; AURKB; AIK2; AIM1; ARK2; STK12
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Application Data:
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Calculated MW:
39280 Da
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(LEFT) Western blot analysis of anti-STK12 Antibody (N-term) (Cat.#AP7240a) in HepG2 cell line lysates (35ug/lane). STK12 (arrow) was detected using the purified Pab.
(MIDDLE) The STK12 polyclonal antibody(Cat. #AP7240a) is used in Western blot to detect STK12 in mouse brain tissue lysate.
(RIGHT) Western blot analysis of AURKB (arrow) using rabbit polyclonal Aurora-B (ARK/STK12) Antibody (N-term) (Cat.#AP7240a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AURKB gene (Lane 2). |
(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
(RIGHT)Formalin-fixed and paraffin-embedded human testis tissue reacted with STK12 Antibody (N-term) (Cat.#AP7240a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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Data Rewards Program
>
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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