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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP7563c
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WB, IHC, FC, E
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H More
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RB14696
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_004337, P42574
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CASP3.
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Other Names:
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Caspase-3 [Precursor]; CASP-3; Apopain; Cysteine protease CPP32; Yama protein; CPP-32; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12; CASP3
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Application Data:
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Calculated MW:
31608 Da
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Western blot analysis of anti-CASP3 Pab (Cat.#AP7563c) in NCI-H460 cell line lysates (35ug/lane).CASP3 (arrow) was detected using the purified Pab (1:60 dilution). |
(LEFT) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP3 antibody (Center) (Cat.#AP7563c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
(RIGHT) Flow cytometric analysis of NCI-H460 cells using Caspase-3 (CASP3) Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Product Citations:
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Data Rewards Program
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
| Flow cytometric | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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