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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP7807a
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WB, IHC, E, IF†
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H, M More
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RB3736
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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P37023
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ACVRL1.
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Other Names:
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SKR3, Activin receptor-like kinase 1, ALK-1, TGF-B superfamily receptor type I, TSR-I
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Application Data:
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Calculated MW:
56124 Da
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Western blot of ACVRL1 Pab (Cat. #AP7807a). TOP LEFT: Mouse heart tissue lysate. TOP RIGHT: Western blot analysis of ACVRL1 (arrow) using rabbit polyclonal ACVRL1 Antibody (N-term) (Cat. #AP7807a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACVRL1 gene (Lane 2) (Origene Technologies).
BUTTOM: human chondrocytes (C28/I2 cells), transfected with empty vector (lane 1, 3) or ACVRL1(lane 2, 4). RIPA lysis buffer, 20 ug/lane of protein, primary antibody dilution 1:1000, blocking solution is 5% milk in TBST (lane 1 and 2), 5% BSA in TBST (lane 3 and 4). Data courtesy of Kenneth Finnson, Montreal General Hospital. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Product Citations:
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- Yu, Ming-Jiun, et al. Mol. Cell. Proteomics 2006 Nov 01;5(11):2131-2145. (Applications: IF)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
† Application validated by third-party citation.
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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