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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP7810h
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WB, IHC, E
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H
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RB10948
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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P15056
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S728 of human BRAF.
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Other Names:
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B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1
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Application Data:
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Calculated MW:
84490 Da
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Western blot analysis of BRAF (arrow) using rabbit polyclonal BRAF Antibody (S728) (RB10948). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF Antibody (S728) (Cat.#AP7810h), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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Data Rewards Program
>
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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