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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP8016c
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WB, IHC, E
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H More
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RB11623
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_006209, Q14CW1
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the the center region of human PI3KCA.
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Other Names:
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Phosphatidylinositol-4;5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3-kinase p110 subunit alpha; PtdIns-3-kinase p110; PI3K; PIK3CA;
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Application Data:
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Calculated MW:
124284 Da
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(LEFT)Western blot analysis of anti-PIK3CA Antibody (Center) Pab (Cat.#AP8016c) in K562 cell line lysates (35ug/lane). PIK3CA (arrow) was detected using the purified Pab (1:60 dilution) (RIGHT)Western blot analysis of anti-PI3KCA Antibody (Center) Pab (Cat.#AP8016c) in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane). PI3KCA (arrow) was detected using the purified Pab (1:60 dilution). |
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody (Center)(Cat.#AP8016c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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Data Rewards Program
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:50-100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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