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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP8151b
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WB, IHC, E
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H, M More
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RB5284
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_002750, Q52M43
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PRKR.
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Other Names:
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Interferon-induced, double-stranded RNA-activated protein kinase; Interferon-inducible RNA-dependent protein kinase; p68 kinase; P1/eIF-2A protein kinase; EIF2AK2; PKR
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Application Data:
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Calculated MW:
62094 Da
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(LEFT)The anti-PRKR Pab (Cat. #AP8151b) is used in Western blot to detect PRKR in mouse uterus tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).
(RIGHT)Western blot analysis of EIF2AK2 (arrow) using PRKR Antibody (C-term) (Cat.#AP8151b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF2AK2 gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Product Citations:
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Data Rewards Program
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
| Flow cytometry | |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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