|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AM1002a
|
Mouse
|
SD20505C
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
P08581
|
6AT203
|
Mouse IgG1
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, E
|
H, M
|
155526 Da
|
|
Target/Specificity:
|
|
This monoclonal antibody is generated from mice immunized with purified recombinant protein encoding the catalytic domain of human Met.
|
|
Other Names:
|
|
HGF receptor
|
|
Application Data:
|
Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgents different anti-Met monoclonal antibodies that are Cat# AM1001-1005. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# AM1000). |
|
|
Background:
|
|
MET/HGFR is a receptor for hepatocyte growth factor, with tyrosine-protein kinase activity. The MET/HGFR Type I membrane protein contains 1 Sema domain and consists of a heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. In the fully processed c-Met product, the alpha subunit is extracellular, and the beta subunit has extracellular, transmembrane, and tyrosine kinase domains as well as sites of tyrosine phosphorylation. Two isoforms for the protein have been described. Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. MET is overexpressed in a significant percentage of human cancers and is amplified during the transition between primary tumors and metastasis. Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC), also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance.
|
|
Background References:
|
- Furge KA, et al. (2000) Oncogene 19(49):5582-9.
- Dean M, et al. (1985) Nature 318(6044), 385-388
- Dean M, et al. (1987) Mol. Cell. Biol. 7(2), 921-924
- Park,M., et al. (1987) Proc. Natl. Acad. Sci. U.S.A. 84(18), 6379-6383
- Bottaro DP, et al. (1991) Science 251(4995), 802-804
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
|
|
Format:
|
|
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
