|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AM1007a
|
Mouse
|
SD40628
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
|
9E10
|
Mouse IgG1
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, E
|
N/A
|
|
|
Target/Specificity:
|
|
KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen.
|
|
Other Names:
|
|
Tag from c-Myc protein
|
|
Application Data:
|
Myc-tagged recombinant protein was expressed in COS7 cells. 20 ug of cell lysate was loaded onto a gel to test the Myc tag antibody (AM1007a). |
|
|
Background:
|
|
Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-Myc monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.
|
|
Background References:
|
- Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19.
- Sells MA and Chernoff J. (1995) Gene, 152:187-9.
|
|
Product Citations:
|
- Evgeny A. Zemskov, et al. J. Cell Sci., Sep 2007; 120: 3188 - 3199. (Applications: WB)
- Hang, Jun, et al. J. Biol. Chem. 2005 Jun 24;280(25):23675-23683. (Applications: WB)
|
|
Application Notes:
|
| ELISA | 1:3,000 |
| Western blotting | 1:2,000 |
|
|
Format:
|
|
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
