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Catalog #
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Source:
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Lot #
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Size:
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AM1021a
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Mouse
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CA080306A
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0.1 ml
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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Crude ascites
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NP_001092, P60709
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4E8H3
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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41737 Da
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Target/Specificity:
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ACTB recombinant protein is used to produce this monoclonal antibody.
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Other Names:
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Actin, cytoplasmic 1; Beta-actin; ACTB
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Application Data:
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Western blot analysis of anti-Beta-actin Monoclonal Antibody (Cat. #AM1021a) in HL60 and Jurkat cell line lysates (35ug/lane). Beta-actin(arrow) was detected using the Mab ascites (1:25000 dilution). |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Beta-actin Monoclonal Antibody (Cat.#AM1021a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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beta-Actin is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
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Background References:
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- Villebeck,L., Biochemistry 46 (44), 12639-12647 (2007)
- Avizienyte,E., Exp. Cell Res. 313 (15), 3175-3188 (2007)
- Bouldin,A.A., Muscle Nerve 35 (2), 254-258 (2007)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| Internal Control | |
| Western blotting | 1:5000~20000 |
| Immunohistochemistry | 1:1000 |
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Format:
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Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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