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Catalog #
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Source:
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Lot #
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Size:
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AM1029a
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Mouse
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SD060601A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_031548
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27AT381
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Mouse IgG1
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M
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22080 Da
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Target/Specificity:
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This monoclonal antibody is generated from mice immunized with a KLH conjugated synthetic peptide corresponding to sequences in Ser155 region of mouse Bad and residue Ser155 is phosphorylated.
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Other Names:
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Bcl-associated death promoter
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Application Data:
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Western analysis of cell extracts from 293 cells transfected with Flag-Bad, using Phospho-mBad-Ser155 Antibody. |
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Background:
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Apoptosis is a critical aspect of cellular function and is implicated in a broad range of human diseases, from cancer to neurodegenerative and cardiovascular diseases. Intrafamilial Bcl-2 protein contacts are thought to regulate key aspects of apoptosis. The corruption of apoptotic instructions is associated with a large subset of human diseases, ranging from cancer and cardiovascular disease to neurodegenerative diseases, and name just a few. Understanding regulation of apoptosis is necessary to constructing a strategy for pharmaceutical intervention. The BH3 domain of Bcl-2 family members has been identified as key to the Bcl-2 regulatory function, although details are not fully elucidated.
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Background References:
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- Chiang, C.W., et al., Mol. Cell. Biol. 23(18):6350-6362 (2003).
- Danial, N.N., et al., Nature 424(6951):952-956 (2003).
- Ranger, A.M., et al., Proc. Natl. Acad. Sci. U.S.A. 100(16):9324-9329 (2003).
- Benetti, L., et al., J. Virol. 77(11):6567-6573 (2003).
- Ibata-Ombetta, S., et al., J. Biol. Chem. 278(15):13086-13093 (2003).
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Product Citations:
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Application Notes:
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| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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