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Catalog #
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Source:
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Lot #
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Size:
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AM1110b
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Mouse
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SD50717A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_036244, Q9ULW0
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19AT953.286.93
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Mouse IgG1
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M
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85653 Da
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Target/Specificity:
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This monoclonal antibody is generated from mice immunized with a collection of antigenic peptides from different regions of P100.
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Other Names:
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TPX2, C20orf1, C20orf2, DIL2, HCA519, p100, Protein FLS353, Hepatocellular carcinoma-associated antigen 519, Targeting protein for Xklp2, Restricted expression proliferation associated protein 100, Differentially expressed in cancerous and noncancerous lung cells 2, DIL-2
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Application Data:
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Western analysis of extracts from Jurkat cells using P100 antibody. |
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Background:
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The nuclear protein p100 is a proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M. Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. The full-length cDNA encodes a 747-amino acid protein with a putative ATP/GTP-binding site motif. RT-PCR analysis demonstrated strong expression of in lung carcinoma cell lines but not in normal lung tissues. Expression was also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney. P100 is also correlated to cancer prognosis.
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Background References:
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- Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
- Heidebrecht, H.J., et al., Mol. Cancer Res. 1(4):271-279 (2003).
- Garrett, S., et al., Curr. Biol. 12(23):2055-2059 (2002).
- Gruss, O.J., et al., Nat. Cell Biol. 4(11):871-879 (2002).
- Kufer, T.A., et al., J. Cell Biol. 158(4):617-623 (2002).
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Product Citations:
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Application Notes:
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| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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