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Catalog #
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Source:
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Lot #
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Size:
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AM1120a
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Mouse
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SDG051122A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_062376, Q9Z239
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41AT858.235
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Mouse IgG1
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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M
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10323 Da
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Target/Specificity:
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This monoclonal antibody is generated from mice immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S68 of mouse PLM.
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Other Names:
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phospholemman, FXYD1
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Application Data:
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Western blot analysis of anti-PLM Mab (Cat. #AM1120a) in 293 cells transfected with a plasmid encoding PLM. |
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Background:
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PLM (FXYD1)is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. PLM may be phosphorylated by several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity.
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Background References:
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- Crowell, K.J., et al., Biochim. Biophys. Acta 1645(1):15-21 (2003).
- Sweadner, K.J., et al., Genomics 68(1):41-56 (2000).
- Mounsey, J.P., et al., J. Biol. Chem. 275(30):23362-23367 (2000).
- Chen, L.S., et al., Genomics 41(3):435-443 (1997).
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Product Citations:
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Application Notes:
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| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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