|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AM1130a
|
Mouse
|
SD050907A
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
NP_006008, O43490
|
32AT1672
|
Mouse IgG1
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, FC, E
|
H
|
97201 Da
|
|
Target/Specificity:
|
|
This monoclonal antibody is generated from mice immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of AC133.
|
|
Other Names:
|
|
Prominin-like protein 1, Antigen AC133, CD133 antigen, PROM1, PROML1, CD133
|
|
Application Data:
|
Western blot analysis of cell lysates from Y79 cell lines using AC133 antibody am1130a. Immunofluorescence analysis of Bone Marrow Mononuclear Cells stained with monoclonal CD133. Image courtesy of Rick Cohen from the Coriell Institute for Medical Research (NJ, USA). |
|
|
Background:
|
|
The CD133 gene codes for a pentaspan transmembrane glycoprotein. The CD133 antigen appears to belong to a new molecular family of 5-TM proteins, as the characterization of the CD133 antigen and prominin in the mouse were the first descriptions of a 5-TM glycoprotein structure. This 'family' includes members from several different species (which may be homologs) including human, mouse, rat, fly, and worm. The 5-TM structure includes an extracellular N-terminus, two short intracellular loops, two large extracellular loops and an intracellular C-terminus CD133 was initially shown to be expressed on primitive hematopoietic stem and progenitor cells and retinoblastoma. CD133 has since been shown to be expressed on hemangioblasts, and neural stem cells as well as on developing epithelium. Expression patterns for CD133 generally mimic those of the murine prominin molecule, although CD133 antigen has not yet been demonstrated on adult epithelial tissue. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors. Phenotypically, CD133 positive cells in blood and marrow are CD34 bright, with CD34 dim CD71 bright cells being negative for CD133 expression. Many leukemias express CD133 as well as CD34 , but some investigators have noted leukemic blasts which are CD133+ and CD34 negative. No natural ligand has yet been demonstrated for the CD133 molecule, and its function in hematopoietic tissue is unknown.
|
|
Background References:
|
- Giebel, B., et al., Blood 104(8):2332-2338 (2004).
- Torrente, Y., et al., J. Clin. Invest. 114(2):182-195 (2004).
- Shmelkov, S.V., et al., Blood 103(6):2055-2061 (2004).
- Yu, Y., et al., J. Biol. Chem. 277(23):20711-20716 (2002).
- Corbeil, D., et al., Biochem. Biophys. Res. Commun. 285(4):939-944 (2001).
|
|
Product Citations:
|
- Gashaw, Isabella, et al. Mol. Hum. Reprod. 2007 Oct 01;13(10):721-727.
|
|
Application Notes:
|
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunofluorescence | 0.180555556 |
|
|
Format:
|
|
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
