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Catalog #
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Source:
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Lot #
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Size:
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AM1485a
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Mouse
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SH081216M SG080828A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_078950, Q9H9Z2
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55CT58.12.1
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IgG1 Kappa
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, IF, E
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H
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22743 Da
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Target/Specificity:
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LIN28A recombinant protein is used to produce this monoclonal antibody.
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Other Names:
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Lin-28 homolog A; Zinc finger CCHC domain-containing protein 1; LIN28; CSDD1; LIN28A; ZCCHC1
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Application Data:
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Western blot analysis of LIN28 recombinant protein by anti-LIN28A Monoclonal Antibody (Cat.#AM1485a). LIN28 (arrow) was detected using the purified Mab. |
(LEFT) Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28A Monoclonal Antibody (Cat.#AM1485a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
(RIGHT) Immunofluorescence analysis of LIN28A Monoclonal Antibody with paraffin-embedded human testis tissue. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence. |
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Background:
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LIN28A acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. It is essential for skeletal muscle differentiation through the translational up-regulation of IGF2 expression.
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Background References:
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- Piskounova,E., J. Biol. Chem. 283 (31), 21310-21314 (2008)
- Wu,L., Mol. Cell. Biol. 25 (21), 9198-9208 (2005)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:10000 |
| Western blotting | 1:200~2000 |
| Immunohistochemistry | 1:50~200 |
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Format:
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Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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