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Catalog #
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Source:
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Lot #
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Size:
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AM1817a
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Mouse
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CA080728A
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0.1 ml
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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Crude ascites
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NP_110430, Q676U5
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54CT27.2.6
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IgG1κ
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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68265 Da
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Target/Specificity:
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Purified His-tagged ATG16L1 protein(Fragment) was used to produced this monoclonal antibody.
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Other Names:
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Autophagy-related protein 16-1; APG16-like 1; ATG16L1; APG16L
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Application Data:
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Western blot analysis of anti-ATG16L1 Monoclonal Antibody (Cat.#AM1817a) by ATG16L1 recombinant protein (Fragment). ATG16L1 (Fragment) protein (arrow) was detected using the ascites Mab. (1:2000) |
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Background:
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The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
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Background References:
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- Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
- Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
- Greenberg JT. Dev Cell. 8(6):799-801. (2005)
- Levine B. Cell. 120(2):159-62. (2005)
- Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:4000 |
| Western blotting | 1:2000 |
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Format:
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Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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