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Catalog #
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Source:
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Lot #
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Size:
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AM3111a
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Mouse
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SD070404A
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0.1 ml
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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Crude ascites
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NP_997006, Q13480
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1AT1979
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Mouse IgG1
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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76616 Da
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Target/Specificity:
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This antibody is generated from mice immunized with a GST fusion protein encoding full length human GAB1.
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Other Names:
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GRB2-associated-binding protein 1; GRB2-associated binder 1; GAB1;
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Application Data:
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Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42°C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37°C for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with anti-Gab1 antibody. Data courtesy of Dr. GS Feng at The Burnham Institute. |
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Background:
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The Grb2-associated binder 1 (Gab1) is a multisubstrate scaffold/adapter protein that contains a pleckstrin homology domain at the NH2 terminus and multiple tyrosyl residues and proline-rich motifs for association with src homology 2 (SH2)-containing and SH3-containing proteins. Gab1, as well as its close relatives Gab2 and Gab3, serves to couple signaling between cell surface receptors for growth factors and cytokines with a variety of downstream targets, including Shp2 tyrosine phosphatase, phosphatidylinositol 3-kinase (PI3K), and phospholipase C-gamma. Ablation of the Gab1 gene in mice resulted in embryonic death in homozygous mutants, with developmental defects in the placenta, heart, and skin. Biochemical analysis of Gab1-/- fibroblast cells suggested a positive role of Gab1 in coupling the signal relay between cytokine receptors and the ERK pathway.
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Background References:
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- Podar, K., et al., J. Biol. Chem. 279(20):21658-21665 (2004).
- Ren, Y., et al., J. Biol. Chem. 279(9):8497-8505 (2004).
- Kapoor, G.S., et al., Mol. Cell. Biol. 24(2):823-836 (2004).
- Nakaoka, Y., et al., Circ. Res. 93(3):221-229 (2003).
- Holgado-Madruga, M., et al., Mol. Cell. Biol. 23(13):4471-4484 (2003).
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Product Citations:
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- Sun, Yingqing, et al. Mol. Cell. Biol. 2004 Feb 15;24(4):1531-1539. (Applications: WB)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Immunohistochemistry | 1:200~1000 |
| Immuniprecipitation | 2-5 ug |
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Format:
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Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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