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Catalog #
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Source:
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Lot #
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Size:
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AM6406a
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Mouse
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CA080523A
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0.1 ml
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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Ascites
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NP_115785, Q9BXM7
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38CT18.7
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H,M
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62769 Da
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Target/Specificity:
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Recombinant PINK1 protein was used to produced this monoclonal antibody.
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Other Names:
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PTEN induced putative kinase 1; protein kinase BRPK; PINK1; Serine/threonine kinase PINK1, mitochondrial [Precursor]
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Application Data:
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(LEFT) Western blot analysis of anti-PINK1 Monoclonal Antibody (AM6406a) in mouse brain tissue lysates. PINK1(arrow) was detected using the ascites Mab. (dilution 1:500)
(RIGHT) Western blot analysis of PINK (arrow) using mouse monoclonal PINK antibody(Ascites). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PINK gene (Lane 2) (Origene Technologies) (1:2000) |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PINK1 Monoclonal Antibody (Cat.#AM6406a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Defects in PINK1 are the cause of autosomal recessive early-onset Parkinson's disease 6 (PARK6). Six novel pathogenic PINK1 mutations suggest that PINK1 may be the second most common causative gene next to parkin in parkinsonism with the recessive mode of inheritance. Strong evidence indicates that, although important in mendelian forms of Parkinson's disease (PD), PINK1 does not influence the cause of sporadic nonmendelian forms of PD.
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Background References:
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- Rogaeva,E.,et al. Arch. Neurol. 61 (12), 1898-1904 (2004)
- Hatano,Y., et al. Ann. Neurol. 56 (3), 424-427 (2004)
- Healy,D.G., et al. Ann. Neurol. 56 (3), 329-335 (2004)
- Valente,E.M., et al. Science 304 (5674), 1158-1160 (2004)
- Nakajima,A., et al. Cancer Lett. 201 (2), 195-201 (2003)
- Unoki,M. and Nakamura,Y. Oncogene 20 (33), 4457-4465 (2001)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:500~2000 |
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Format:
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Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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