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Catalog #
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Source:
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Lot #
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Size:
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AM7718a
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Mouse
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SD41014A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P12931
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17AT28
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Mouse IgG1
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Applications:
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Reactivity:
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MW (kDa):
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WB, IP, IHC, E
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H, M
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59703 Da
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Target/Specificity:
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This antibody is generated from mouse immunized with a recombinant protein of full length human SRC.
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Other Names:
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Proto-oncogene tyrosine-protein kinase Src, p60-Src, c-Src
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Application Data:
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The anti-Src Mab (Cat. #AM7718a) is used in Western blot to detect Src in Jurkat cell lysate. |
FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (AM7718a) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 °C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.
Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD. |
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Background:
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The gene for SRC is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. SRC, the protein encoded by this gene, is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in SRC could be involved in the malignant progression of colon cancer.
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Background References:
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- Lopez, R.G., et al., J. Biol. Chem. 278(42):41316-41325 (2003).
- Horn, S., et al., Oncogene 22(46):7170-7180 (2003).
- Hakam, A., et al., Dig. Dis. Sci. 48(10):1972-1978 (2003).
- Ito, H., et al., Surgery 134(2):221-226 (2003).
- Bouchard, M.J., et al., J. Virol. 77(14):7713-7719 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
| Immunoprecipitation | 2 ug/test |
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Format:
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Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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