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Catalog #
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Source:
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Lot #
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Size:
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AM8108a
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Mouse
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SD31223B
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001269, O15111
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61AT277
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Mouse IgG1
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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84653 Da
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Target/Specificity:
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This antibody was raised using purified His-tagged recombinant full length human IKK alpha.
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Other Names:
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Inhibitor of nuclear factor kappa-B kinase alpha subunit, I kappa-B kinase alpha, IkBKA, IKK-A, IkappaB kinase, I-kappa-B kinase 1, IKK1, Conserved helix-loop-helix ubiquitous kinase, Nuclear factor NFkappaB inhibitor kinase alpha, NFKBIKA
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Application Data:
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The anti-IKKalpha Mab (Cat. #AM8108a) is used in Western blot to detect IKKalpha in HeLa cell lysate. |
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Background:
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IKK alpha is a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the
ubiquination pathway, thereby activating the transcription factor.
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Background References:
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- Xiao, G., et al., J. Biol. Chem. 279(29):30099-30105 (2004).
- Krappmann, D., et al., Mol. Cell. Biol. 24(14):6488-6500 (2004).
- Hironaka, N., et al., Neoplasia 6(3):266-278 (2004).
- O'Mahony, A.M., et al., J. Biol. Chem. 279(18):18137-18145 (2004).
- Gustin, J.A., et al., J. Biol. Chem. 279(3):1615-1620 (2004).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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