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Catalog #
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Source:
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Lot #
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Size:
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AM8109a
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Mouse
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SD40218B
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001547, O14920
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62AT216
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Mouse IgG1
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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86563 Da
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Target/Specificity:
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This antibody was raised using purified His-tagged recombinant full length human IKK beta.
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Other Names:
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Inhibitor of nuclear factor kappa B kinase beta subunit, I-kappa-B-kinase beta, IkBKB, IKK-B, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB
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Application Data:
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The anti-IKKbeta Mab (Cat. #AM8109a) is used in Western blot to detect IKKbeta in Jurkat cell lysate. |
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Background:
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IKK beta is a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
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Background References:
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- Carcamo, J.M., et al., Mol. Cell. Biol. 24(15):6645-6652 (2004).
- Xiao, G., et al., J. Biol. Chem. 279(29):30099-30105 (2004).
- Mattioli, I., et al., J. Immunol. 172(10):6336-6344 (2004).
- Viemann, D., et al., Blood 103(9):3365-3373 (2004).
- Caposio, P., et al., J. Virol. 78(6):3190-3195 (2004).
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Product Citations:
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- Panopoulos, Andreas, et al. J. Biol. Chem. 2005 Jan 28;280(4):2912-2923. (Applications: WB)
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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