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PRMT4 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP1006a Rabbit SH020306G 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml XP_032719 RB0032 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 65723 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PRMT4.
Other Names:
Protein Arginine N-Methyltransferase 4, CARM1
Application Data:

Western blot analysis of PRMT4 polyclonal antibody (Cat. #AP1006a) in whole HL60 cell lysate: PRMT4 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PRMT4 antibody (C-term)(Cat.#AP1006a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Background:
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
Background References:
  1. Wada K, et al. Biochim Biophys Acta. 2002. 1591:1.
  2. Cimato TR, et al. J Neurosci Res. 2002. 67:435.
  3. Frankel A, et al. J Biol Chem. 2002. 277:3537.
  4. Brahms H, et al. RNA. 2001. 7:1531.
  5. Pelletier M, et al. Mol Biochem Parasitol. 2001. 118:49.
  6. Belyanskaya LL, et al. J Biol Chem. 2001. 276:18681.
  7. Rho J, et al. J Biol Chem. 2001. 276:11393.
  8. Scorilas A, et al. Biochem Biophys Res Commun. 2000. 278:349.
  9. Frankel A, et al. J Biol Chem. 2000. 275:32974.
  10. Zhang X, et al. EMBO J. 19:3509.
  11. Tang J, et al. J Biol Chem. 1998. 273:16935.
Product Citations:
Application Notes:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry:1:10~50
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.