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Catalog #
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Source:
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Lot #
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Size:
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AP1015a
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Rabbit
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SH020610S
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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RB0415
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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N/A
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Target/Specificity:
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KLH conjugated synthetic peptide encoding 3xBtag (CQYPALTQYPALTQYPALT) was used as antigen.
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Other Names:
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Application Data:
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Western blot analysis of anti-B tag Pab (Cat. #AP1015a)in B-tagged recombinant protein bacterial lysate. |
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Background:
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Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. BTag is an epitope tag composed of a 6 residue peptide, QYPALT, derived from the highly conserved region of the major core protein, VP7, of bluetongue (BT) viruses.
Abgent's anti-BTag polyclonal antibody provides a simple solution to detect the expression of a B-tagged protein in cells.
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Background References:
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- Wang LF, Yu M, White JR, Eaton BT. BTag: a novel six-residue epitope tag for surveillance and purification of recombinant proteins. Gene. 1996 Feb 22;169(1):53-8.
- du Plessis DH, Wang LF, Jordaan FA, Eaton BT. Fine mapping of a continuous epitope on VP7 of bluetongue virus using overlapping synthetic peptides and a random epitope library. Virology. 1994 Jan;198(1):346-9.
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Product Citations:
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Application Notes:
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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