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Catalog #
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Source:
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Lot #
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Size:
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AP1017a
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Rabbit
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SH020611H
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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RB0420
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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N/A
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Target/Specificity:
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KLH conjugated synthetic peptide encoding the Protein-C epitope tag (CEDQVDPRLIDGK) was used as antigen.
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Other Names:
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Application Data:
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Western blot analysis of anti-Protein-C tag Pab (Cat. #AP1017a)in Protein-C-tagged recombinant protein bacterial lysate. |
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Background:
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Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The epitope tag composed of a 12 residue peptide, EDQVDPRLIDGK , is derived from Protein-C, a vitamin K-dependent serine protease that regulates blood coagluation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids.
Abgent's anti-Protein-C polyclonal antibody provides a simple solution to detect the expression of a Protein-C epitope-tagged protein in cells.
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Background References:
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- Lichty JJ, Malecki JL, Agnew HD, Michelson-Horowitz DJ, Tan S. Comparison of affinity tags for protein purification. Protein Expr Purif. 2005 May;41(1):98-105.
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Product Citations:
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Application Notes:
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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