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Catalog #
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Source:
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Lot #
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Size:
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AP1018a
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Rabbit
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SH071107A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_079033, Q9H9B1
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RB13017
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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138254 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human EHMT1.
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Other Names:
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Histone H3-K9 methyltransferase 5; H3-K9-HMTase 5; Euchromatic histone-lysine N-methyltransferase 1; Eu-HMTase1; G9a-like protein 1; GLP1; Lysine N-methyltransferase 1D; EHMT1; EUHMTASE1; KIAA1876; KMT1D
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Application Data:
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Western blot analysis of EHMT1 (arrow) using rabbit polyclonal EHMT1 Antibody (N-term) (Cat# AP1018a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EHMT1 gene (Lane 2) (Origene Technologies). |
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Background:
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EHMT1, also known as EUHMTASE1, is a histone methyltransferase. This protein methylates 'Lys-9' of histone H3 in vitro. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle.
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Background References:
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- Ogawa H.,Science 296:1132-1136(2002).
- Ota T.,Nat. Genet. 36:40-45(2004).
- Nagase T.,DNA Res. 8:85-95(2001).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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