|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP1021b
|
Rabbit
|
SH021220P
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
O14717
|
RB1850
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, IHC, E
|
H, M
|
44596 Da
|
|
Target/Specificity:
|
|
"This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the human Dnmt2 sequence within aa 300~400. This antibody will recognize the splice isoforms A, B, and C."
|
|
Other Names:
|
|
DNMT2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, M.HsaIIP, PuMet
|
|
Application Data:
|
Western blot analysis of anti-Dnmt2 Pab (Cat. #AP1021b) in Jurkat cell lysate (Lane A) and mouse liver tissue lysate (Lane B). Dnmt2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
|
|
|
Background:
|
|
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a protein with similarity to DNA methyltransferases, but this protein does not display methyltransferase activity. The protein strongly binds DNA, suggesting that it may mark specific sequences in the genome. Alternative splicing results in multiple transcript variants encoding different isoforms.
|
|
Background References:
|
- Hermann, A., et al., J. Biol. Chem. 278(34):31717-31721 (2003).
- Franchina, M., et al., Int. J. Biochem. Cell Biol. 33(11):1104-1115 (2001).
- Dong, A., et al., Nucleic Acids Res. 29(2):439-448 (2001).
- Yoder, J.A., et al., Hum. Mol. Genet. 7(2):279-284 (1998).
- Van den Wyngaert, I., et al., FEBS Lett. 426(2):283-289 (1998).
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
