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Catalog #
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Source:
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Lot #
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Size:
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AP1022a
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Rabbit
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SH060921A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001073893, O15054
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RB10077
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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180411 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human JMJD3.
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Other Names:
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JmjC domain-containing protein 3; Jumonji domain-containing protein 3; JMJD3; KIAA0346;; KDM6B
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Application Data:
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Western blot analysis of anti-JMJD3 N-term Pab (AP1022a) in Hela cell line lysates. JMJD3 N-term(arrow) was detected using the purified Pab. |
Formalin-fixed and paraffin-embedded human brain tissue reacted with JMJD3 N-term (Cat.#AP1022a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
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Background References:
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Product Citations:
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- Hai Long, et al. Lupus, Dec 2009; 18: 1327 - 1328. (E-pub)
- Makoto Ishii, et al. Blood, 8 October 2009, Vol. 114, No. 15, pp. 3244-3254. (Applications: WB)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:250~500 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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