|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP1039c
|
Rabbit
|
SH030213E
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
NP_003916
|
RB2383
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, E
|
H
|
66050 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MBD4.
|
|
Other Names:
|
|
Methyl-CpG-binding Domain 4
|
|
Application Data:
|
Western blot analysis of anti-MBD4 Pab (Cat. #AP1039c) in HeLa cell lysate. MBD4 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
|
|
Background:
|
|
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and the MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene.
|
|
Background References:
|
- Evertson, S., et al., Anticancer Res. 23(4):3569-3574 (2003).
- Yamada, T., et al., Cancer Lett. 181(1):115-120 (2002).
- Schlegel, J., et al., Oncol. Rep. 9(2):393-395 (2002).
- Petronzelli, F., et al., J. Biol. Chem. 275(42):32422-32429 (2000).
- Petronzelli, F., et al., J. Cell. Physiol. 185(3):473-480 (2000).
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
