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Catalog #
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Source:
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Lot #
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Size:
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AP1051d
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Rabbit
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SA051021D
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_002098, NP_005315, P84243
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RB2673
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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15273 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S10 of human Histone H3.
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Other Names:
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Histone H3; H3F3A; H3F3B
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Application Data:
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Western blot analysis of Histone H3 (N-term) (Cat.#AP1051d) in HL60 cell line lysates (35ug/lane). Histone H3 (arrow) was detected using the purified Pab. |
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Background:
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. The protein targeted by this antibody is a replication-independent member of the histone H3 family.
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Background References:
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- Marzluff, W.F., et al., Genomics 80(5):487-498 (2002).
- Albig, W., et al., Hum. Genet. 101(3):284-294 (1997).
- Albig, W., et al., Genomics 40(2):314-322 (1997).
- Albig, W., et al., Genomics 10(4):940-948 (1991).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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