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Catalog #
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Source:
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Lot #
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Size:
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AP1073a
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Rabbit
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SH040607I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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CAI13326, Q5SZD8
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RB5746
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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27702 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SETDB1.
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Other Names:
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Histone H3-K9 methyltransferase 4; H3-K9-HMTase 4; SET domain bifurcated 1; ERG-associated protein with SET domain; ESET;; KMT1E
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Application Data:
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Western blot analysis of anti-SETDB1 Pab(Cat. #AP1073a) in Jurkat tissue lysate. SETDB1(arrow) was detected using the purified Pab. |
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Background:
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The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.
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Background References:
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- Ichimura, T., et al., J. Biol. Chem. 280(14):13928-13935 (2005).
- Sarraf, S.A., et al., Mol. Cell 15(4):595-605 (2004).
- Wang, H., et al., Mol. Cell 12(2):475-487 (2003).
- Schultz, D.C., et al., Genes Dev. 16(8):919-932 (2002).
- Yang, L., et al., Biochem. J. 369 (PT 3), 651-657 (2003) (): ().
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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