|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP1076b
|
Rabbit
|
SH040608K
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
NP_061833, Q14749
|
RB5760
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, E
|
H
|
32611 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GNMT.
|
|
Other Names:
|
|
Glycine N-methyltransferase
|
|
Application Data:
|
Western blot analysis of anti-GNMT Pab (Cat. #AP1076b) in CEM cell line tissue lysate (35ug/lane).GNMT(arrow) was detected using the purified Pab (1:60 dilution). |
|
|
Background:
|
|
Glycine N-methyltransferase catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. GNMT acts as an enzyme to regulate the ratio of S-adenosylmethionine to S-adenosylhomocysteine (AdoHcy) and participates in the detoxification pathway in liver cells.
|
|
Background References:
|
- Augoustides-Savvopoulou, P., et al., J. Inherit. Metab. Dis. 26(8):745-759 (2003).
- Tseng, T.L., et al., Cancer Res. 63(3):647-654 (2003).
- Luka, Z., et al., Hum. Genet. 110(1):68-74 (2002).
- Strausberg RL, et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
- Mudd, S.H., et al., J. Inherit. Metab. Dis. 24(4):448-464 (2001).
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
