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Catalog #
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Source:
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Lot #
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Size:
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AP1078b
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Rabbit
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SH040609E
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_066564, Q8N1A2
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RB5766
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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93836 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GCN5L2.
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Other Names:
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Histone acetyltransferase GCN5; hsGCN5; STAF97;
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Application Data:
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Western blot analysis of anti-GCN5L2 Pab (Cat. #AP1078b) in CEM cell line tissue lysate (35ug/lane).GCN5L2(arrow) was detected using the purified Pab (1:60 dilution). |
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Background:
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GCN5L2 functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. This protein has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles
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Background References:
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- Kikuchi, H., et al., Gene 347(1):83-97 (2005).
- Gangisetty, O., et al., Oncogene 23(14):2559-2563 (2004).
- Lusic, M., et al., EMBO J. 22(24):6550-6561 (2003).
- Fulco, M., et al., Mol. Cell 12(1):51-62 (2003).
- Liu, X., et al., J. Biol. Chem. 278(22):20405-20412 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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