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Catalog #
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Source:
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Lot #
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Size:
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AP1079a
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Rabbit
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SH040609G
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_003633, O14929
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RB5768
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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49513 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human HAT1.
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Other Names:
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Histone acetyltransferase type B catalytic subunit; KAT1
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Application Data:
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Western blot analysis of KAT1 (HAT1) Antibody (N-term) (Cat. #AP1079a) in HL60 cell line lysates (35ug/lane). HAT1 (arrow) was detected using the purified Pab. |
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Background:
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Histone acetylation, particularly of histone H4, has been proposed to play an important role in replication-dependent nucleosome assembly. The HAT1 protein contains D, A, and B motifs, which are present in many N-acetyltransferases, including those that acetylate substrates other than histones. The HAT1 holoenzyme consists of 2 subunits: the catalytic 46-kD HAT1 and the accessory p46. The p46 subunit stimulated the activity of HAT1 and bound to core histones. The HAT1 holoenzyme acetylated newly synthesized but not nucleosomal histone H4 at lys5 and lys12, and, to a lesser extent, histone H2A at lys5. HAT1 and p46 polypeptides are located in the nucleus of S-phase cells. HAT1 may play a role in telomeric silencing.
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Background References:
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- Gronroos, E., et al., Mol. Cell 10(3):483-493 (2002).
- Makowski, A.M., et al., J. Biol. Chem. 276(47):43499-43502 (2001).
- Cheung, P., et al., Mol. Cell 5(6):905-915 (2000).
- Verreault, A., et al., Curr. Biol. 8(2):96-108 (1998).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blot | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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