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Catalog #
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Source:
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Lot #
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Size:
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AP1105a
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Rabbit
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SH040527L
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q9UQL6
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RB5650
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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121991 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human HDAC5.
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Other Names:
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Histone deacetylase 5; HD5; Antigen NY-CO-9; KIAA0600
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Application Data:
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The anti-HDAC5 Pab (Cat. #AP1105a) is used in Western blot to detect HDAC5 in 293 cell lysate. |
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Background:
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Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with the HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This protein is thought to be associated with colon cancer.
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Background References:
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- Berger, I., et al., J. Biol. Chem. 278(20):17625-17635 (2003).
- Zhang, C.L., et al., Mol. Cell. Biol. 22(20):7302-7312 (2002).
- Lemercier, C., et al., J. Biol. Chem. 277(24):22045-22052 (2002).
- Huang, Y., et al., Cancer Res. 62(10):2913-2922 (2002).
- Grozinger, C.M., et al., Proc. Natl. Acad. Sci. U.S.A. 97(14):7835-7840 (2000).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Flow cytometry | |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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